Deoxynucleotide-polymerizing Enzymes of Calf Thymus Gland
نویسندگان
چکیده
Replication of polydeoxyadenylate and polydeoxythymidylate catalyzed by the high molecular weight DNA polymerase proceeds only in the presence of a complementary oligodeoxynucleotide. The oligodeoxynucleotide initiator chain must be greater than 6 nucleotides for poly(dA) replication and greater than 5 nucleotides for poly(dT) replication. The initiator oligonucleotide is incorporated into product chains. When a multiplicity of initiator chains are present (more than 1 per template) then several chains grow simultaneously on each template. The structure and molecular weight distribution of the products may be predicted from the initiator to template ratio.
منابع مشابه
Deoxynucleotide-polymerizing enzymes of calf thymus gland. II. Properties of the terminal deoxynucleotidyltransferase.
and the ratio of polymer nucleotide to monomer at equilibrium (pH 7.0, 35’) is about 99 when X and Y = adenine. Puriue deoxyribonucleoside triphosphate polymerizations proceed readily in 8 mM Mgf+ at low buffer strengths (40 mr+r). For deoxyadenosine triphosphate, under these conditions, K, = 1.1 x 10-‘&r and I’,,, varies from about 0.03 to 0.30 pmole mm+ mg+ with various oligodeoxynucleotide i...
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The term polymerase has been applied to the deoxyribonucleoside triphosphate-polymerizing enzyme isolated from Escherichia coli (1). Enzymes with similar activity have been purified from regenerating rat liver (2) and calf thymus gland (3). An ample and varied supply of such polymerases is desirable for studies on the mechanism of deoxyribonucleic acid synthesis. The present work describes a pa...
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The polymerization of deoxynucleoside triphosphates, catalyzed by terminal deoxynucleotidyl transferase from calf thymus gland, is strongly inhibited by various metal chelators. The reaction appears to be first order with respect to enzyme, deoxynucleoside triphosphate, and initiator, indicating that there is only one catalytic center for each enzyme molecule. The presence of metal chelator doe...
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